STRUCTURE OF TYPE 5 ADENOVIRUS
نویسندگان
چکیده
منابع مشابه
Structure of Type 5 Adenovirus
Type 5 adenovirus was purified by fluorocarbon (freon 113) treatment followed by banding in a CsCl equilibrium density gradient. This method permitted separation of virus from normal host cell materials and virus-specific soluble antigens. Virus banded in CsCl with a mean bouyant density of 1.3349 gm/cm(3). The three virus-specific soluble antigens (group- and type-specific antigens and toxin) ...
متن کاملObservations on the Fine Structure of Type 5 Adenovirus
Since the first observation with the electron microscope of intranuclear virus-like particles in sections of tissue culture cells infected with adeno-virus (1), numerous later studies of similar material have established certain morphological features of this agent (2-5). The particles have been reported to be round or ellipsoid and an inner body and outer limiting membrane have been described ...
متن کاملInactivation of adenovirus type 5 by caustics.
Adenovirus shows significant promise as a vehicle for transfer of therapeutic genes into humans. Based on the importance of this viral vector, it is critical that adequate decontamination procedures are implemented during its large-scale production in multiproduct manufacturing facilities to prevent cross-product contamination and to reduce the risk of personnel exposure. Liquid decontamination...
متن کاملCharacterization of type 5 adenovirus fiber protein.
Type 5 adenovirus fiber protein was purified and subjected to chemical characterization. Equilibrium sedimentation ultracentrifugation analysis indicated that the intact fiber has a molecular weight of approximately 183,000. Denaturation and chemical analyses implied that the fiber consists of three polypeptide chains, each of about 61,000 mol wt. Mapping of tryptic peptides and electrophoretic...
متن کاملComplementation of human adenovirus type 5 ts mutants by human adenovirus type 12.
Temperature-sensitive mutants of type 5 adenovirus belonging to eight complementation groups were complemented in mixed infection by type 12 adenovirus, whereas mutants of 7 other groups were not enhanced. In some crosses, phenotypic mixing took place. No evidence of recombination between type 5 ts mutants and type 12 was found.
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1963
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.118.2.307